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Archive > Volume 35 Issue 4 > 2019,35(4):22-29. DOI:10.13982/j.mfst.1673-9078.2019.4.004 Prev Next

Lipid-Modulating Effects of Visceral Lipids from Haliotis discus hannai on HepG2 Cells

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    Abstract:
    In this study, the effects of the visceral lipids from Haliotis discus hannai (HDHL) on lipid metabolism of human hepatoma cell line, HepG2 cells, were investigated. The HDHL fatty acid composition, HepG2 cell activity, and the content of intracellular cholesterol and triglycerides as well as the mRNA expression of fatty acids metabolism-related genes were examined. It was found that the unsaturated fatty acids in HDHL accounted for 59.50% of the total fatty acids, of which polyunsaturated fatty acids accounted for 29.71%. After an incubation of HDHL and HepG2 cells for 24 h and 48 h, respectively, HDHL at 0~240 μg/mL had no toxic effect on HepG2 cells, whereas, HDHL at 30~240 μg/mL could significantly reduce the total cholesterol content of HepG2, with HDHL at 30~120 μg/mL causing a significant decrease in the content of intracellular triglycerides. The qPCR results revealed that in HepG2 cells, HDHL could significantly reduce the mRNA expression of fatty acid synthesis-related genes SREBP1c, ACC1 and FAS as well as fatty acid transport and absorption-related gene CD36, while increasing the expression of mitochondrial fatty acid oxidation gene CPT1. Therefore, HDHL might regulate cell lipid metabolism through inhibiting the synthesis and transportation of fatty acids while enhancing the oxidation of fatty acids in mitochondria. This study provides a theoretical basis for the development of lipid-regulating functional foods.

    Keywords:
    human hepatoma cell HepG2 ;  Haliotis discus hannai ;  visceral lipids ;  lipid metabolism

    [1] 董诗婷,陈弘培,赵慧,等.海洋生物功能性脂类的研究进展[J].农产品加工,2017,7:59-64
    DONG Shi-ting, CHEN Hong-pei, ZHAO Hui, et al. The reasearch progress of marine functional lipids [J]. Farm Products Processing, 2017, 7: 59-64
    [2] Wang S, Pessah M, Infante J F, et al. Lipid and lipoprotein metabolism in Hep G2 cells [J]. Biochimica et Biophysica Acta, 1988, 961: 351-363
    [3] Shimomura I, Shimano H, Horton J D, et al. Differential expression of exons 1a and 1c in mRNAs for sterol regulatory element binding protein-1 in human and mouse organs and cultured cells [J]. The Journal of Clinical Investigation, 1997, 99: 838-845
    [4] Kohjima M, Enjoji M, Higuchi N, et al. The effects of unsaturated fatty acids on lipid metabolism in HepG2 cells [J]. In Vitro Cellular & Developmental Biology-Animal, 2009, 45: 6-9
    [5] Mitsuyoshi H, Yasui K, Harano Y, et al. Analysis of hepatic genes involved in the metabolism of fatty acids and iron in nonalcoholic fatty liver disease [J]. Hepatology Research : the Official Journal of the Japan Society of Hepatology, 2009, 39: 366-373
    [6] Wan Y, Liu L Y, Hong Z F, et al. Ethanol extract of Cirsium japonicum attenuates hepatic lipid accumulation via AMPK activation in human HepG2 cells [J]. Experimental and Therapeutic Medicine, 2014, 8: 79-84
    [7] Krockenberger M, Dombrowski Y, Weidler C, et al. Macrophage migration inhibitory factor contributes to the immune escape of ovarian cancer by down-regulating NKG2D [J]. Journal of Immunology, 2008, 180: 7338-7348
    [8] Arendt B M, Comelli E M, Ma D W, et al. Altered hepatic gene expression in nonalcoholic fatty liver disease is associated with lower hepatic n-3 and n-6 polyunsaturated fatty acids [J]. Hepatology, 2015, 61: 1565-1578
    [9] 刘裕,高燕翔,冉莉,等.不同膳食脂肪酸对大鼠肝脏SREBP -1c基因表达和非酒精性脂肪肝发生的影响[J].第三军医大学学报,2016,38:2002-2007
    LIU Yu, GAO Yan-xiang, RAN Li, et al. Effects of dietary fatty acids on expression of gene sterol-regulatory elementary binding protein-1c and occurrence of non-alcoholic fatty liver disease in rats [J]. Journal of Third Military Medical University, 2016, 38: 2002-2007
    [10] 时皎皎,糜漫天,韦娜,等.不同膳食脂肪酸构成对大鼠肝脏脂代谢相关基因表达的影响[J].重庆医学,2012,41:1252-1254
    SHI Jiao-jiao, MI Man-tian, WEI Na, et al. Effect of fatty acid compositions in different diets on expression of lipid metabolism related genes in rat liver [J]. Chongqing Medicine, 2012, 41: 1252-1254
    [11] Tillander V, Bjorndal B, Burri L, et al. Fish oil and krill oil supplementations differentially regulate lipid catabolic and synthetic pathways in mice [J]. Nutrition & Metabolism, 2014, 11: 20
    [12] 胡晓倩.海参皂苷对脂质代谢的影响及其机制研究 [D].青岛:中国海洋大学,2010
    HU Xiao-qian. Study on effcets and mechanism of saponins of sea cucumber on lipid metabolism [D]. Qingdao: Ocean University of China, 2010 [13] 童国玉.固醇调节元件结合蛋白1c的研究进展[J].国外医学.内分泌学分册,2002:328-331
    TONG Guo-yu. Research progress of sterol regulatory element-binding protein 1c [J]. Foreign Medical Sciences (Section of Endocrinology), 2002: 328-331
    [14] Zamora-Valdes D, Ponciano-Rodriguez G, Chavez-Tapia N C, et al. The endocannabinoid system in chronic liver disease [J]. Annals of Hepatology, 2005, 4: 248-254
    [15] Mao J, Demayo F J, Li H, et al. Liver-specific deletion of acetyl-CoA carboxylase 1 reduces hepatic triglyceride accumulation without affecting glucose homeostasis [J]. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103: 8552-8557
    [16] Bargut T C, Frantz E D, Mandarim-de-Lacerda C A, et al. Effects of a diet rich in n-3 polyunsaturated fatty acids on hepatic lipogenesis and beta-oxidation in mice [J]. Lipids, 2014, 49: 431-444
    [17] Liu X, Xue Y, Liu C, et al. Eicosapentaenoic acid-enriched phospholipid ameliorates insulin resistance and lipid metabolism in diet-induced-obese mice [J]. Lipids in Health and Disease, 2013, 12: 109
    [18] Hu X Q, Wang Y M, Wang J F, et al. Dietary saponins of sea cucumber alleviate orotic acid-induced fatty liver in rats via PPARalpha and SREBP-1c signaling [J]. Lipids in Health and Disease, 2010, 9: 25
    [19] Jeon H J, Choi H S, Lee Y J, et al. Seapolynol extracted from ecklonia cava inhibits adipocyte differentiation in vitro and decreases fat accumulation in vivo [J]. Molecules, 2015, 20: 21715-21731
    [20] Poirier Y, Antonenkov V D, Glumoff T, et al. Peroxisomal β-oxidation-A metabolic pathway with multiple functions [J]. Biochimica et Biophysica Acta, 2006, 1763: 1413-1426
    [21] Gutieres S, Damon M, Panserat S, et al. Cloning and tissue distribution of a carnitine palmitoyltransferase I gene in rainbow trout (Oncorhynchus mykiss) [J]. Comparative Biochemistry and Physiology B, 2003, 135: 139-151
    [22] Nyman L R, Tian L, Hamm D A, et al. Long term effects of high fat or high carbohydrate diets on glucose tolerance in mice with heterozygous carnitine palmitoyltransferase-1a (CPT-1a) deficiency: Diet influences on CPT1a deficient mice [J]. Nutrition & Diabetes, 2011, 1: e14
    [23] Ferramosca A, Conte A, Burri L, et al. A krill oil supplemented diet suppresses hepatic steatosis in high-fat fed rats [J]. PloS One, 2012, 7: e38797
    [24] Koonen D P, Glatz J F, Bonen A, et al. Long-chain fatty acid uptake and FAT/CD36 translocation in heart and skeletal muscle [J]. Biochim Biophys Acta, 2005, 1736: 163-180
    [25] Koonen D P, Jacobs R L, Febbraio M, et al. Increased hepatic CD36 expression contributes to dyslipidemia associated with diet-induced obesity [J]. Diabetes, 2007, 56: 2863-2871
    [26] Miquilenacolina M E, Limacabello E, Sanchezcampos S, et al. Hepatic fatty acid translocase CD36 upregulation is associated with insulin resistance, hyperinsulinaemia and increased steatosis in non-alcoholic steatohepatitis and chronic hepatitis C [J]. Gut, 2011, 60: 1394-1402
    [27] 黄康.绿原酸对大鼠肝脏脂肪代谢调节机制研究[D].长沙:湖南农业大学,2014
    HUANG Kang. Regulatory mechanism of Chlorogenic Acid on Hepatic Lipid Metabolism in Rats [D]. Changsha: Hunan Agricultural University, 2014
    [28] Li X, Zhao M, Fan L, et al. Chitobiose alleviates oleic acid-induced lipid accumulation by decreasing fatty acid uptake and triglyceride synthesis in HepG2 cells [J]. Journal of Functional Foods, 2018, 46: 202-211
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