Lipid-Modulating Effects of Visceral Lipids from Haliotis discus hannai on HepG2 Cells
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Abstract:
In this study, the effects of the visceral lipids from Haliotis discus hannai (HDHL) on lipid metabolism of human hepatoma cell line, HepG2 cells, were investigated. The HDHL fatty acid composition, HepG2 cell activity, and the content of intracellular cholesterol and triglycerides as well as the mRNA expression of fatty acids metabolism-related genes were examined. It was found that the unsaturated fatty acids in HDHL accounted for 59.50% of the total fatty acids, of which polyunsaturated fatty acids accounted for 29.71%. After an incubation of HDHL and HepG2 cells for 24 h and 48 h, respectively, HDHL at 0~240 μg/mL had no toxic effect on HepG2 cells, whereas, HDHL at 30~240 μg/mL could significantly reduce the total cholesterol content of HepG2, with HDHL at 30~120 μg/mL causing a significant decrease in the content of intracellular triglycerides. The qPCR results revealed that in HepG2 cells, HDHL could significantly reduce the mRNA expression of fatty acid synthesis-related genes SREBP1c, ACC1 and FAS as well as fatty acid transport and absorption-related gene CD36, while increasing the expression of mitochondrial fatty acid oxidation gene CPT1. Therefore, HDHL might regulate cell lipid metabolism through inhibiting the synthesis and transportation of fatty acids while enhancing the oxidation of fatty acids in mitochondria. This study provides a theoretical basis for the development of lipid-regulating functional foods.
