Effect of Green Tea Extract on the Nonalcoholic Steatohepatitis Induced by MCD Diet
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Abstract:
To evaluate the effect of green tea extract on the NASH mice -induced by MCD diet and its possible mechanism, forty C57BL/6 mice were randomly divided into four groups: control group (MCS diet), MCD group (MCD diet), MCD diet supplemented with green tea extract (0.15 g/kg/day or 0.30 g/kg/day). After 8-weeks administration, blood and liver were harvested. The levels of serum ALT, AST, IL-6 and TNF-α were measured. The levels of hepatic TG and TC were also measured. The oil red stained was used to evaluate the lipid accumulation area in liver. Additionally, the liver tissues were stained with hematoxylin-eosin to evaluate the hepatic steatosis, inflammation and the ballooning according to the non-alcoholic fatty liver disease activity score. Western blot was used to analyze the protein expression levels of GRP78 and LC3. Results showed that, compared to MCD model, administration of green tea extract could significantly decrease the levels of serum ALT、AST、IL-6 and TNF-α, with the largest decrease rate of 38%, 45%, 46% and 74%, respectively. In addition, result of oil red O staining indicated that the extract also could obviously attenuate the accumulated fat in the liver. The extract could decrease the levels of hepatic TG and TC, with the largest decrease rate of 44% and 37%, when compared to MCD group. Result of H&E staining showed that the score of steatosis, lobular inflammation, hepatic ballooning was significantly reduced by green tea extract in C57/BL6 mice subjected to MCD diet. The expression level of GRP78 was markedly restrained while that of LC3-II was increased by administration of green tea extract. Green tea extract has beneficial effects against NASH-induced by MCD diet. The regulations of GRP78 and LC3-II might be involved in the mechanisms of action of green tea extract.
