Abstract:We have analyzed the antioxidant ability of four main antioxidants (gallic acid, rutin, vitamin C (Vc), glutathione (GSH)) of mango using vitro mimetic experiment and determined the influence of different antioxidant combinations on the scavenging capacities of hydroxyl radical (·OH), superoxide anion (O2-·) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). It showed that the highest scavenging rate of ·OH was gallic acid + rutin + GSH + Vc, which could reach 99%, however, the single Vc group was the lowest, 27% lower than the highest group. The highest scavenging rate of O2-· was GSH + Vc, which reached 87%, the complex group of gallic acid + GSH + rutin was only 4%. The rutin+GSH complex group had the highest clearance rate of DPPH (35%), while gallic acid + Vc was merely 2.50% on DPPH clearance rate. It indicated that the antioxidant capacity of the antioxidants may act alone or in combination, and the best effect on free radicals was GSH + Vc.
