Identification of a Spoilage Bacterium S from Eggs with Tolerance to Egg White Protein and De novo Sequencing Analysis of the Strain
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    Abstract:

    A Gram-positive bacterial strain named S was isolated from egg by traditional microbial culture method. The strain was identified by morphological observation, biochemical analysis and 16S rDNA. The phylogenetic tree was also constructed. The tolerance of the strain to egg white environment was investigated by inhibition zone and survival test. The whole genome of the strain was sequenced using De novo system and a draft map was obtained. The results showed that the physiological and biochemical characteristics of the strain were similar to Dermacoccus genus, and the homology of 16S rDNA sequence with Dermacoccus abyssi (Accession number AY894323) reached 99%. The strain S was identified as Dermacoccus abyssi. The strain can grow in the test tube containing egg white or lysozyme. There was no bacteriostatic zone showed in the pore with egg white or lysozyme. Functional annotations of protein-coding genes showed that there were abundant genes involved in membrane transport, ion binding, amino acid and carbohydrate metabolism and energy conversion. It was speculated that these function clusters might be implicated in the strain to survival in the antimicrobial environment of egg white. The tolerance of the strain to egg white and lysozyme provided favorable conditions for its survival in eggs.

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History
  • Received:November 30,2018
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  • Online: April 03,2019
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