Determination of 10 Kinds of Synthetic Colorants in Variety of Food Substrates by SPE-HPLC
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Abstract:
To establish a separation and analysis method for the simultaneous determination of 10 kinds of synthetic colorants, such as lemon yellow, sunset yellow, new red, amaranth, indigo, carmine, tempting red, bright blue, red peony and acid red. Comparing the separation of 10 colorants with Eclipse XDB-C18, Venusil XBP C18 and Symmetry C18 columns, the recovery rates of three solid phase extraction cartridges on Sep-Pak Plus QMA, ProElut PWA-2 and Cleanert PWAX-SPE for ten kinds of Synthetic colorants were characterized for qualitative and quantitative purposes. This method could realize the separation and analysis of ten synthetic colorants in 27 min. It has a good linear relationship in the range of 0.20~50.00 μg/mL (R2>0.999). The recovery ratio of the standard mixture was 69.71%~103.80% (n=6) in the samples, relative standard deviation range was 0.72%~9.41% (n=6). The pretreatment of this method was simple and efficient, with high accuracy and good reproducibility. The recovery rate, detection limit and precision could meet the requirements of national standards. The false negative rate was suitable for large-scale matrix foods in grassroots laboratories.
