Rapid Detection for Sulfonamide Antibiotics Residues in Swine Urine Using TLC-SERS
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Abstract:
A rapid detection method for sulfonamide antibiotics residues in swine urine was developed based on thin layer chromatography (TLC) and surface enhanced Raman spectroscopy (SERS) technology. Position samples were made by antibiotics being added to negative swine urine. Chloroform-ethanol-petroleum ether (1:1:2) was used as a mobile phase to separate antibiotics from swine urine. The excitation wavelength was 785 nm. Gold colloidal was used to enhance Raman signals. Coupled with Density functional theory, sulfadiazine characteristic peaks 877 cm-1 and 994 cm-1, sulfamethazine characteristic peak 661 cm-1, sulfathiazole characteristic peaks 862 cm-1 and 1127 cm-1 were found and used to qualitatively and quantitatively analyze antibiotics residues in swine urine. The minimum detection amounts of sulfadiazine, sulfamethazine and sulfathiazole in swine urine were 0.1, 0.1 and 0.2 mg/L, respectively. And TLC can achieve the separation of mixed antibiotics in swine urine. The characteristic peaks of each antibiotic are easily distinguished when the concentration of three mixed antibiotics in swine urine was 0.01 μg. TLC-SERS technology showed to be suitable for rapid detection of antibiotic residues in pig urine.
