The Use of COI Sequence for Non-directional Screening to Detect Adulteration in Mutton
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Abstract:
This study investigated non-directional screening of other animal source materials incorporated in mutton by using COI sequence, while distinguishing goat meat and sheep meat. DNA barcoding technology was used to screen the mitochondrial genome cytochrome gene fragment of six sheep breeds (lines), and the selected primers were used to amplify the COI fragments of seven animal source samples to determine whether or not the primer can effectively distinguish the lamb from other animal source samples. Using LCOI490/HCO2198, F1-1/R1-1, and ITS3/ITS4 as primers, PCR amplification was performed on the genomic DNAs of 36 blood samples (3 goat species and 3 sheep species), with the PCR product obtained using F1-1/R1-1 as the primer being clear and reproducible. F1-1/R1-1 was used as the primer in the amplification of the COI fragment of chicken, pork, donkey meat, beef, Laiwu black goat meat, mutton sheep meat and duck meat samples, and then the amplified fragments of the seven kinds of meat samples were sequenced. The sequence homology between goat and sheep was only 36.62%, and the sequence difference among other breeds was also obvious The results showed that F1-1/R1-1 can be used as a special primer to distinguish effectively between mutton meat and meat from other animal sources, and also between goat meat and sheep meat samples.
