Study on Optimization of Enrichment of Staphylococcus aureus by Bare Magnetic Particles
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Abstract:
The adsorption efficiency of bare magnetic particles for different concentrations of Staphylococcus aureus and for mixed bacteria was studied. And the method was applied to the detection of S. aureus in beef samples. The quantity of beads, the broth volume and the reaction time were optimized for enrichment the bacteria, to examine if the method of Real-Time Fluorescent Quantitative PCR is suitable for measuring the content of bacteria. The results showed that the bare magnetic particles had high adsorption rate to S. aureus in the broth, with the content of 102 ~107 cfu/mL in the culture broth. The adsorption rate was over 95%. The DNA from the bacterium cells enriched by 10mg beads and 5mL culture broth for 10 minutes could be detected by Fluorescent Quantitative PCR. The DNA extracted from S. aureus was detected by Fluorescent Quantitative PCR after enriched by beads in beef broth at the concentration of 102 cfu/mL. Moreover, the DNA extracted from S. aureus was tested after enriched by beads from the mixture broth with Escherichia coli. The method could be used for enrichment and detection of S. aureus in food.
