Analysis of Delphinin and Preparation of Delphindin from Purple Eggplant Peel
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Abstract:
In this work, the low price peel of purple eggplant was used as material to obtain expensive high purification delphinidin monomer. The anthocyanin in purple eggplant peel was qualitatively analyzed by spectrum and mass spectrometry, and 4delphindins were identified. Then the optimized conditions for extraction delphinidin were investigated. Result showed that optimal extraction solvent was 60% ethanol with 1% hydrochloric acid; ratio of solid to liquid was 1:20. AB-8 macroporous resin which eluted with ethanol was used for initial purification of anthocyanin, the concentration of 30% ethanol elute was the optimal, and the UV absorption value of 530 nm at this concentration was 0.49. Subsequently, the delphinidin glycoside was acid hydrolyzed, and delphinidin aglycone was purified by AB-8 macroporous resin and C-18 resin. Finally, delphinidin powder with purity more than 98% was obtained. It provides a practical method for separating and purifying delphinidin pigments.
