Abstract:Ultrasonic-assisted extraction optimized by single factor experiment and response surface methodology (RSM) was implemented to extract polysacchrides from the fruiting body of Agaricus bitorquis (Quél.) Sacc. Results showed that the optimal condition was ultrasonic wave power 130 W, liquid /solid ratio 1:40, extracting time 15 min. The anti-hypoxia effect of crude polysacchrides extracted by the optimal condition was evaluated by national standard method. The death time, survival time and the gasp time of mice under atmospheric hypoxia, sodium nitrite poisoning and acute cerebral ischemic hypoxia were determined. Compared with the blank control and the positive control (Rhodiola rosea), variance analysis showed that the anti-hypoxia effect of polysacchrides prepared by the optimal extraction condition were significant (P<0.05). Three test results were determined to be positive. Compared to blank, crude polysaccharides significantly extended the death time, survival time and the gasp time by 76.60%, 50.33% and 144.96%, respectively. The death time, survival time and the gasp time of the crude polysaccharides treatment were also 19.20%, 34.20% and 43.07% longer than the positive control, respectively. In conclusion, this study revealed that the crude polysaccharides from the fruiting body of Agaricus bitorquis (Quél.) Sacc have potent anti-hypoxia effects.
