Study on Amygdalin and Prunasin of Amygdalus pedunculata Pall Kernel and Meal by HPLC
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
To clear hazardous substances from degradation products more quickly and effectively in Amygdalus pedunculata Pall meal and its by-products, high performance liquid chromatography (HPLC) was established, the amygdalin and prunasin could be determined simultaneously. When methanol was used to extract amygdalin and prunasin in Amygdalus pedunculata Pall kernel and Amygdalus pedunculata Pall meal, the suitable determination conditions were as follows: Agilent ZORBAX SB-C18 column (5 μm, 4.6 mm×250 mm), mobile phase was 20% methanol and 80% water, the flow rate was 1.00 mL/min, the detection wavelength was 210 nm, column temperature 30 ℃. The peaks of amygdalin and prunasin were isolated well; the linear range for amygdalin was 0.50~300 μg/mL (R2=0.9999), while the prunasin was 0.30~100 μg/mL (R2=0.9999); the average recovery of amygdalin of Amygdalus pedunculata Pall kernel and meal were 87.10%~96.19% (RSD=4.64%~7.83%), 98.32%~107.99% (RSD=1.44%~3.36%), respectively; while the average recovery of prunasin were 88.65%~103.10% (RSD=3.03%~7.55%), 107.58%~117.60% (RSD=1.45%~2.26%), respectively. The method is accurate and reliable, and can be used in the determination of amygdalin and prunasin in Amygdalus pedunculata Pall kernel and meal.
