Isolation, Identification and Screening of Oleaginous Microalgae
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Abstract:
Microalgae oil is an important raw material for biodiesel production, and also one of the raw materials for the production of unsaturated fatty acids. In order to explore the potential of the oleaginous microalgae for future research and applications, we collected water samples from the East Lake of the South China University of Technology in Guangzhou and studied the growth and oil accumulation characteristics of microalgae. A total of six green algae were obtained. The identification of 18S rDNA suggested that microalgae DH1, DH2 and DH6 belong to the genus Chlorella, while strains DH3, DH4 and DH5 belong to the genus Scenedesmus. After analysis, the phylogenetic tree of six microalgal strains was constructed. The determination of intracellular oil content revealed that microalgae DH1、DH2 and DH6, especially DH2 and DH6 could accumulate oil very well. After 25 days of cultivation, the maximum biomass (cell number) of DH2 and DH6 reached 2.14 ×107/mL and 2.74×107/mL, respectively, and the oil yield reached 103.89 mg/L and 131.69 mg/L, respectively, as well as the total lipid contents were all both over 30%. These results suggest that microalgal strains DH2 and DH6 are potentially the ideal oleaginous microalgae which can be used for biodiesel production.
