Screening of High-yield Nucleic Acid Saccharomyces cerevisiae Strain by Atmospheric and Room-temperature Plasma (ARTP) Technique
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Abstract:
As an important biomolecule, ribonucleic acid (RNA) can be degraded into nucleotides, nucleosides and bases which exhibit a great potential in wide applications. Saccharomyces cerevisiae is widely used in food industry and generally regarded as safe are the preferred source of nucleic acid. In this study, the cells were repeatedly exposed under atmospheric and room temperature plasma (ARTP), with the aim to screen high-yield nucleic acid strains with potassium chloride sensitivity. A mutant strain Y17aM3 was successfully obtained. In shake flask test with molasses as carbon source, Y17aM3 contained 112 mg-RNA/g-DCW RNA, 39% higher than that of the untreated control. The optimal inoculation quantity of Y17aM3 to produce RNA was 10%.The optimal pH and temperature of fermentation was 5.5 and 26 ℃, respectively. When culturing with optimal condition, it was found that adding phosphoric acid increased RNA content to 119 mg-RNA/g-DCW, and adding peptone increased RNA content to 122 mg-RNA/g-DCW. The results not only demonstrate the effectiveness of using ARTP mutation strategy for the industrial microorganism breeding, but also can greatly reduce the production cost of nucleotide-based food additives.
