Effects of Waterless Keep Alive Time on Physiological and Biochemical Index of Ictalurus punctatus
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Abstract:
The effect of waterless keep alive time on biochemical index and muscle quality of Ictalurus punctatus was investigated. Optimum keep alive conditions of carbon dioxide and survival rate at different keep-alive time without water were studied in this experiment. The quality indexes of blood biochemistry and fish protein, fat and water content under different keep alive time (5, 7, 9, 11 h) were analyzed before and after waterless keep alive and 24 h after recovery. The results showed that the optimum concentration of CO2 and temperature of waterless keep alive are 500~550 mg/L and 4~8 ℃. The anesthetized Ictalurus punctatus with carbon dioxide were kept alive at 6 ℃ for 5, 7, 9, 11 and 13 h respectively and then transferred to clear water to recover for 24 h. The survival rate is 100%, 100%, 95%, 45% and 20% respectively. As the keep alive time increases, water loss and water holding time have no significant changes (p<0.05). The pH, protein and crude fat in muscle were lower than those in control group, but the content of muscle glycogen was significantly decreased (p<0.05). The activities of urea nitrogen (BUN), creatinine (Cr), pyruvate kinase (PK), lactate dehydrogenase (LDH) and transaminase (GOT) in waterless keep alive group were significantly higher than those in the control and recovery groups(p<0. 05). The activity of GOT and BUN is most closely related to the keep alive time. When the keep alive time was 9 h, GOT increased from 18.48μmol/L to 25.09 μmol/L, BUN increased from 13.18 mmol/L to 16.44 mmol/L, and their activities increased significantly (p<0.05). After recovery in clear water for 24 h, the blood indexes of Cr, BUN and GOT basically recovered to the levels of the control group(p>0.05). When the keep alive time was 11h, their activities of Cr, BUN and GOT increased significantly (p<0.05), which was 52.41%, 25.48% and 48.24% higher than those of the control group respectively. Conclusion: The best keep alive time anesthetized with carbon dioxide is 9 h. The metabolism of Ictalurus punctatus is slow and the toxin can’t be discharged in vitro with the prolongation of the waterless keep alive time, and it will cause irreversible damage to the kidney and liver. The results in this study would provide information for studying the application of waterless keep alive transportation of Ictalurus punctatus.
