Purification and Thermal Stability of Anthocyanin from Hibiscus sabdariffa L.
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Abstract:
In this study, the conditions for the purification of anthocyanins from Hibiscus sabdariffa L. and the thermal stability of these anthocyanins in the presence of different stabilizers were investigated using roselle anthocyanins as raw materials. The results showed that the optimized conditions for the purification of anthocyanins were: Loading concentration, 600 mg/L; Equilibrium time, 3 h; Loading volume,183 mL; eluent, 60% aqueous ethanol; elution flow rate, 1 mL/min. The color value of the lyophilized powder of purified anthocyanins was 43.10±2.17, with a recovery of (83.62±5.72)% and anthocyanin content of 216.50±1.83 mg/g. The degradation of three systems containing roselle anthocyanins together with 1.0% sodium alginate, carboxymethylcellulose (CMC) or β-cyclodextrin at 80, 90 or 100 ℃ all followed the first-order kinetics. The degradation rate constant increased with elevated temperature while the half-life decreased with increasing temperature. β-Cyclodextrin had the potential to delay the degradation of anthocyanins. The degradation rate of anthocyanins became smaller and smaller with an increase of β-cyclodextrin concentration. After heating at 80 ℃ for 150 min, the a* value of the anthocyanin solutions decreased while the b* value increased. An increase of β-cyclodextrin concentration facilitated the maintenance of the redness of anthocyanins, and 1.5% β-cyclodextrin exhibited the most effective protection.
