Comparison of 3M PetrifilmTM Rapid Test Strips and the National Standard Method for Analyzing the Mold and Yeast Species in Beverages
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Abstract:
In this study, 3M PetrifilmTM Rapid Test Strips for yeasts and molds and the national standard method were used in parallel for mold count and yeast count tests for a functional vitamin beverage, to determine whether or not the analysis results obtained by the two methods were significantly different. Five standard strains (Candida albicans, Candida tropicalis, Debaryomyces hansenii, Trichoderma virens and Aspergillus carbonarius) were used for screening. Strain preselection test, repeatability test and specificity test of the rapid test strips, and comparative tests between the rapid test strip method and the national standard method, were performed. The obtained results showed that the strains selected through the preselection test could all grow well in the test samples. Meanwhile, the repeatability test revealed that the results of the second test were within the acceptable range of those of the first test; the specificity test revealed that the rapid test strips method had high specificity. The results of the mold and yeast counts determined by the two comparative methods were statistically analyzed by the F test and t test, and it was found that there was no significant difference between the results obtained by the 3M PetrifilmTM rapid test strip method and the national standard method.
