Extraction Optimization through Response Surface Methodology and Antioxidant Activity of Polyphenols of Nymphaea Hybrid
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Abstract:
In order to explore the best extraction technology and antioxidant activity of polyphenols from Nymphaea hybrid, the optimization of polyphenols extraction conditions was researched by the method of response surface methodology. The influences of extraction temperature, liquid/solid ratio, extraction time, and alcohol concentration on polyphenol extraction yield were researched by single factor experiments. Based on Box-Behnken, four-factor and three-level response surface methodology was carried out to optimize the extraction technology. The antioxidant activity of polyphenols was evaluated using DPPH and ABTS radical scavenging activities and FRAP reducing power. The results found that the optimum conditions for polyphenol extraction were obtained as follows: extraction temperature of 58 ℃, liquid/solid ratio of 48:1 (mL/g), extraction time of 1.9 h, and alcohol concentration of 57%. Under the optimal conditions, extraction rate of N. hybrid polyphenols was 8.28%. The DPPH and ABTS scavenging abilities of N. hybrid polyphenols were 90.22% and 88.01%, respectively. The FRAP value was increased with the polyphenol concentration, exhibiting a dose-dependent effect. These results suggested that N. hybrid polyphenols has an obvious antioxidant activity.
