High-speed Shear-Ultrasonic Combination Extraction of Hypoglycemic Components from the Fruits of Viburnum sargentii Koehne
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Abstract:
In this paper, α-glucosidase and α-amylase inhibition rate were used as indicators to optimize the process of high-speed shear-ultrasonic combination with ethanol assisted extraction of hypoglycemic components from the fruits of Viburnum sargentii Koehne. Regression coefficient and analysis showed that the regression model fitted the actual process well. Based on the results of response surface methodology, the best extraction process parameters were ascertained as follows: the high speed shear dispersing emulsifier’ speed is 18000 r/min, the solid-liquid ratio is 1:19 (g/mL), the extraction temperature is 60 ℃, the ultrasonic time is 40 min, shear cutting time is 120 s, ultrasonic power is 270 W. Under these conditions, the comprehensive inhibition rate was (65.89±1.03)%, the α-glucosidase inhibition rate was (72.11±0.86)%, and the α-amylase inhibition rate was (56.57±1.05)%.The IC50 were 0.844 mg/mL, and 1.422 mg/mL respectively. Comparing enzyme inhibition rate, high-speed shear-ultrasonic combination was 11.39% (p<0.01) higher than the ultrasound method and 13.64% (p<0.01) higher than the high-speed shear method.
