Extraction and Immunomodulatory Activities of Active Components from Chinese Herbs-koji
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Abstract:
In order to evaluate the antioxidant and immunomodulatory activity of a traditional Chinese herbs-koji, the contents of total polyphenols, total flavonoids and polysaccharides were measured. Ultrasound-assisted extraction was used to obtain total polyphenols and total flavonoids. Polysaccharides in Chinese herbs-koji (HKP) were extrated by water extraction and alcohol precipitation. The antioxidant activity of polyphenols and flavonoids were evaluated by the use of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals, hydroxyl radicals, and superoxide anion radicals methods. The potency of HKP on the phagocytosis and cytokine secretion of RAW 264.7 cells was determined by commercial kit. Results showed that the content of total polyphenols in the Chinese herbs-koji was 33.54 mg/g, the total flavonoid content was 10.88 mg/g, and the polysaccharide content was 74.98 mg/g. The extracted polyphenols and flavonoids showed good free-radical scavenging capacity to DPPH radical, superoxide anion radical and hydroxyl radical. Furthermore, the corresponding half inhibitory concentration (IC50) of polyphenols is 18.87 μg/mL, 26.24 μg/mL and 33.31 μg/mL, and for flavonoids is 33.24 μg/mL, 38.97 μg/mL and 33.08 μg/mL. The HKP resulted in a significant increase of lymphocyte proliferation and could also enhance the effect of Con A-induced T lymphocyte proliferation and the stimulation index of LPS-induced B lymphocytes. In addition, the phagocytosis and cytokine secretion of RAW 264.7 cells can be promoted by HKP. The traditional Chinese herbs-koji can be developed and utilized as a health product.
