Effects of Different Extraction Methods on the Composition and Antioxidant Activity of the Polysaccharides in Lilium davidii var. unicolor Salisb.
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Abstract:
Lilium davidii var. unicolor Salisb. was used as the raw material for the investigation on the effects of three extraction methods, microwave-assisted extraction, ultrasonic-assisted extraction, and ultrasonic treatment coupled with enzymatic hydrolysis, on the structure and composition of the obtained polysaccharides (named LPS-M, LPS-C, and LPS-CE, respectively). After precipitation with ethanol, deproteinization and freeze-drying, the three types of polysaccharide samples were characterized by FT- IR, and the result indicated that LPS-M, LPS-C and LPS-CE all had the characteristic absorption peaks of polysaccharides (at 3378, 2931, 1640 and 1060 cm-1). The monosaccharide compositions of LPS-M, LPS-C and LPS-CE were analysed by GC-MS，and the results showed that these polysaccharides had the same types of monosaccharides but at different molar ratios i.e. the molar ratios of the two major components, mannose and glucose, were at 5.2:4.9, 5.1:4.7, and 4.8:5.3, respectively. The analyses of the antioxidant activities by hydroxyl radicals, superoxide radicals, DPPH free radicals and reducing power assays revealed that antioxidant activity of LPS-CE was significantly higher than those of LPS-C and LPS-M. The method of ultrasonic treatment coupled with enzymatic hydrolysis could be used as the preferred extraction method for extracting functional polysaccharides from Lilium davidii var. unicolor Salisb..
