Heat Resistance and Identification of Characteristic Strain of Thermophilic Bacteria from Epidermal Mucus of White Mullet Fish (Opniocepnalus argus var. kimnra)
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Abstract:
To deeply analyze the heat resistance of the characteristic microbes and identifying the characteristic strain in the epidermal mucus of white mullet fish, the carcass mucus of white mullet was heat treated, then the characteristic bacteria in the heat treated mucus were isolated and purified, and their species were identified. By comparing the appearance characteristics and heat resistance of the heated mucus, and morphological features of the characteristic bacteria, the growth curve of the characteristic bacteria and the heat resistance of the strain S3 were investigated. Results showed that the fresh mucus was faint red. The mucus was gradually became light green with the increase of heat temperature. Three kinds of colonies, namely S1, S2, S3, were observed in the culture medium inoculated with the 30 ℃, and S2 and S3 in the culture medium inoculated with the 50 ℃, while only S3 in the culture medium inoculated with the 70 ℃, 90 ℃, boiling treated mucus. No colony was found in the control medium. Further analysis of S1, S2 and S3 bacteria showed that S1 was gram-negative bacterial strains S2 and S3 were gram-positive bacterial strains. The strain S2 and S3 reached the peak of growth when they were cultured for 14h and 16h, respectively. By analyzing 16S rDNA sequence of S1, S2 and S3, it was found that strain S1 belonged to Acinetobacter sp., strain S2 belonged to Kocuriarhizophila sp., and strain S3 belonged to Bacillus sp. Therefore, the thermophilic bacterium inepidermal mucus of white mullet fish is Bacillus subtilis.
