Antifungal Mechanisms of the Active Ingredients Carvacrol of Mosla Chinensis 'Jiangxiangru' against Penicillium Digitatum
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Abstract:
Penicillium digitatum was used as the test fungal species in this study to investigate the antifungal mechanism of the active ingredients of Mosla chinensis 'Jiangxiangru', namely carvacrol. Antifungal activity was evaluated by determination of the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), measuring the antifungal activity of a series of doubling dilution carvacrol samples. The acting mechanism of carvacrol was elucidated by evaluating its effects on mycelial morphology, spore germination, cell membrane permeability, soluble sugar content. Results showed that the MIC and the MFC of carvacrol were 0.125 mg/mL and 0.25 mg/mL, respectively; the morphological changes of mycelium were appeared by treatment with different concentrations carvacrol; furthermore, a lower spore germination rate, elevated cell membrane permeability, reduced soluble sugar were also observed by treatment with different concentrations carvacrol. The antifungal activity of carvacrol may be related to inhibit spore germination in Penicillium digitatum, damage the structure of its cell membrane, facilitate leakage of intracellular contents, reduce nutrient content. Thus, carvacrol exerts an inhibitory effect by inhibiting normal growth and reproduction of mycelia.
