Inhibition of Ethanol-induced Hepatic Steatosis in Human HL7702 Liver Cells by Four Constituents Isolated from Hovenia Acerba
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
To discover the effective substances in Hovenia acerba capable of dispeling the effects of alcohol and protecting the liver, this study examined the effects of four constituents in the ethyl acetate extract from Hovenia acerba on hepatic steatosis. The cells metabolic activity/viability of the control group, model group, or high-/medium-/low-dose group of the four bioactives was measured by the MTT assay. The lipid droplets in the cells were observed by Oil red O staining after ethanol modeling. The levels of cell TG and liver enzymes, ALT and AST, were determined. The optimal concentration of ethanol-induced cells was measured by the MTT assay. It was found that compared with the control group, the cell viability decreased after of 0.9% ethanol modeling for 48 h, along with the observations that Oil red O-stained lipid droplets became more obvious in the cells and the cells became swollen and round. after the exposure of ethanol-treated model cells to each of the four bioactives, a slight increase in the survival rate of the cells was induced by dihydromyricetin and quercetin as compared with the model group; compared with the control group, the TG content and the leakage of ALT and AST of the model group significantly increased (p<0.05). Further, compared with the model group, the application of 0.9% ethanol combined with dihydromyricetin or quercetin on the normal human HL7702 hepatocytes for 48 h caused a significant (p<0.05) decrease in the TG and ALT, AST contents in the liver. Accordingly, the active ingredients in the ethyl acetate phase obtained from Hovenia acerba that were capable of dispeling the effects of alcohol and protecting the liver were mainly dihydromyricetin and quercetin, especially the former.
