The Protection of the CCl4-induced Acute Liver Injury in Mice by Lilium Lancifolium Cyanidin
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The protective effects of Lilium lancifolium cyanidin on the acute liver injury in mice caused by CCl4 were investigated. Fifty mice were randomly divided into blank control group (S), CCl4 group (M), CCl4 + Lilium lancifolium cyanidin low (25 mg/kg, LLC), medium (50 mg/kg, MLC), high (100 mg/kg, HLC) dose groups. Drug groups were given different dose drugs for days. After 2 h in last day, model was developed with the intraperitoneal injection of 0.3 mL 1% CCl4 peanut oil solution. Then, all mice were executed after 24 h, and the serum was collected for the determination of ALT, AST and ALP levels. Liver was weighed and triturated for detecting SOD, CAT and MDA. HE staining was also examined. Western blot was employed to examine the expression of Ｎrf2、HO-1 and NQO1 protein in the liver tissue. Compared with M group, our experimental results showed that Lilium lancifolium cyanidin could significantly reduce the levels of ALT, AST and ALP in serum, increase SOD and CAT enzyme activity of liver tissue and reduce MDA content (p<0.05). At the same time, the Nrf2、HO-1 and NQO1 protein expression of the liver tissue also significantly raised (p<0.05). The results suggest that, Lilium lancifolium cyanidin exhibited a protection against liver damage, and the underlying mechanism may be associated with its mediation of the oxidative stress reaction, antioxidant activities (e.g., scavenging free radical activity, and inhibiting lipid peroxidation activity), and the protection of liver cell membrane structure and function through the Nrf2/OH-1/ NQO1 signaling pathways.
