The protective effects of Lilium lancifolium cyanidin on the acute liver injury in mice caused by CCl4 were investigated. Fifty mice were randomly divided into blank control group (S), CCl4 group (M), CCl4 + Lilium lancifolium cyanidin low (25 mg/kg, LLC), medium (50 mg/kg, MLC), high (100 mg/kg, HLC) dose groups. Drug groups were given different dose drugs for days. After 2 h in last day, model was developed with the intraperitoneal injection of 0.3 mL 1% CCl4 peanut oil solution. Then, all mice were executed after 24 h, and the serum was collected for the determination of ALT, AST and ALP levels. Liver was weighed and triturated for detecting SOD, CAT and MDA. HE staining was also examined. Western blot was employed to examine the expression of Ｎrf2、HO-1 and NQO1 protein in the liver tissue. Compared with M group, our experimental results showed that Lilium lancifolium cyanidin could significantly reduce the levels of ALT, AST and ALP in serum, increase SOD and CAT enzyme activity of liver tissue and reduce MDA content (p<0.05). At the same time, the Nrf2、HO-1 and NQO1 protein expression of the liver tissue also significantly raised (p<0.05). The results suggest that, Lilium lancifolium cyanidin exhibited a protection against liver damage, and the underlying mechanism may be associated with its mediation of the oxidative stress reaction, antioxidant activities (e.g., scavenging free radical activity, and inhibiting lipid peroxidation activity), and the protection of liver cell membrane structure and function through the Nrf2/OH-1/ NQO1 signaling pathways.
[1] Y. Ijiri, R. Kato, M. Sadamatsu, et al. Chronological changes in circulating levels of soluble tumor necrosis factor receptors 1 and 2 in rats with carbon tetrachloride-induced liver injury [J]. Toxicology, 2014, 316: 55-60
[2] Wu S, Yue Y, Tian H, Li Z, Li X, He W, Ding H. Carthamus red from Carthamus tinctorius L. exerts antioxidant and hepatoprotective effect against CCl(4)-induced liver damage in rats via the Nrf2 pathway [J]. J Ethnopharmacol, 2013, 148 (2): 570-578
[3] Silva, G.B. Rodrigues, S.W. Rocha, et al. Inhibition of NF-kappaB activation by diethylcarbamazine prevents alcohol-induced liver injury in C57BL/6 mice [J]. Tissue & cell, 2014, 46: 363-371
[4] Shen, Baode, et al. Hepatoprotective effects of lignans extract from Herpetospermum caudigerum against CCl4-induced acute liver injury in mice [J]. Journal of ethnopharmacology, 2015, 164: 46-52
[5] R. Jeyadevi, T. Sivasudha, A. Rameshkumar, J.M. Harnly, L.-Z. Lin, Phenolic profiling by UPLC–MS/MS and hepatoprotective activity of Cardiospermum halicacabum against CCl4 induced liver injury in Wistar rats [J]. Journal of Functional Foods, 2013, 5: 289-298
[6] 徐立新,严丽军.白桦脂酸对CCl4诱导小鼠急性肝损伤的保护作用及机制研究[J].南京中医药大学学报,2017,33(6):618-622
XU LI-xin, YAN Li-jun. The protective effect of betulinicd acid alleviates CCl4-induced acute liver injury in mice and its related mechanism [J]. J Nanjing Univ Tradit Chin Med, 2017, 33(6): 618-622
[7] 赵冠华,曲敏,胡斯杰,等.硫酸酯化牡蛎多糖对四氯化碳所致小鼠急性肝损伤的影响[J].大连海洋大学学报,2018,33(1):72-76
ZHAO Guan-hua, QU Min, HU Si-jie, et al. Effect of oyster polysaccharide sulfate on acute liver injury induced by carbon tetrachloride in mice [J]. Journal of Dalian Ocean University, 2018, 33(1): 72-76
[8] 李继平,张雪梅,师志强,等.对比研究8批红芪多糖对CCl4所致小鼠肝损伤的药效[J].中成药,2017,9(9):1927-1932
LI Ji-ping, ZHANG Xue-mei, SHI Zhi-qiang, et al. The effects of 8 batches of astragalus polysaccharide on liver injury induced by CCl4 in mice were compared and study [J]. Chinese Traditional Patent Medicine, 2017, 9(9): 1927-1932
[9] López, Caleja,. Prieto, et al. Optimization and comparison of heat and ultrasound assisted extraction techniques to obtain anthocyanin compounds from Arbutus unedoL. Fruits [J]. Food Chemistry, 2018, 264: 81-91
[10] Yari A, Rashnoo S. Optimization of a new method for extraction of cyanidin chloride and pelargonidin chloride anthocyanins with magnetic solid phase extraction and determination in fruit samples by HPLC with central composite design [J]. Journal of Chromatography B, 2017, 1067: 38-44
[11] Ting Wan, Sufan Wang, Mingtong Ye, et al. Cyanidin-3-O-b-glucoside protects against liver fibrosis induced by alcohol via regulating energy homeostasis and AMPK/autophagy signaling pathway [J]. Journal of Functional Foods, 2017, 37: 16-24
