Inhibition of Lipopolysaccharide-induced RAW264.7 Cells Inflammatory Reaction by Total Anthocyanins from Red Cabbage
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Abstract:
The effects of total anthocyanins from red cabbage (RCTA, 1 μg/mL-50 μg/mL) on lipopolysaccharide (LPS, 1 μg/mL) induced inflammatory reaction in RAW264.7 cells were investigated. The cellular levels of nitric oxide (NO) and prostaglandin E2 (PGE2) were determined by commercial kits. Tumor necrosis factor-α (TNF-α), interlukin (IL)-1β, IL-6 and IL-8 were determined by commercial enzyme linked immunosorbent assay (ELISA) kits according the manuscripts. The mRNA expressions of induced nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and inflammatory cytokines related TNF-α, IL-1β, IL-6 and IL-8 were determined by quantitative real time polymerase chain reaction (qRT-PCR) assay. The results indicated that, RCTA significantly reduced the LPS induced secretion of inflammatory mediators (NO and PGE2) and inflammatory cytokines (TNF-α、IL-1β、IL-6 and IL-8) in RAW264.7 cells. The high concentration of RCTA (50 μg/mL) was effectively reduced the mRNA expressions of iNOS (65.80%) and COX-2 (48.28%), and generations of (58.81%) and PGE2 (46.26%) in LPS treated RAW264.7 cells. In addition, RCTA treatment was able to reduce the mRNA expressions of iNOS, COX-2, TNF-α, IL-1β, IL-6 and IL-8, respectively. The results demonstrated that the RCTA exhibited a protective effect to reduce the secretion of inflammatory mediators and cytokines.
