6-shogaol Induces the HT29 Apoptosis and Its Relationship with VEGFR2 Expression in Human Colon Cancer Cell
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Abstract:
In order to unravel the mechanism of the inhibition of colorectal cancer by 6-shogoal, the apoptosis of colon cancer cells HT29 induced by different concentrations of 6-shogoal, as well as its relationship with different expression of VEGFR2 (vascular endothelial cell receptor2) was investigated. The HT29 cells were treated with different concentrations of 6-shogoal (0, 10, 20 and 40 μM) cultured in vitro. The cell morphology changes were observed by the contrast inverted fluorescence microscope, and the cell inhibition rate was determined by CCK8 method. Annexin V-FITC/ PI flow cytometry was used to detect the changes of apoptosis rate and apoptosis cycle. Finally, the expression of VEGFR2 protein was detected by Western-blot. The results showed that as compared with the control group, 6-shogoal could induce HT29 apoptosis in a concentration dependent manner. The number of cell cycle G0/G1 cells decreased from 49.48% to 24.39% and 17.18%, while the number of G2/M cells increased from 3.44% to 34.78% and 49.54%.Thus,the cell cycle was blocked in G2/M stage to inhibit the cell proliferation. Besides, it also inhibited the expression of VEGFR2 to different extents (p<0.05).Therefore, it can be concluded that 6-shogoal can induce HT29 apoptosis and inhibit the proliferation of tumor cells, which may be related to different degrees of inhibition of VEGFR2.
