Detection and Quantification of Goose-derived Materials in Food and Feedstuffs by Droplet Digital PCR
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Abstract:
In this study, a highly precise, quantitative method based on the droplet digital polymerase chain reaction (ddPCR) technique was developed to identify and quantify the goose content in food and feedstuffs. The single-copy Anser cygnoides domesticus genomic scaffold gene was selected as the target gene, a formula for calculating raw meat weight based on target DNA copy number was established. Exclusive specificity was verified using samples from 21 different animal species, and inclusive specificity of goose was tested using nine different breeds. The limit of detection (LOD) and the limit of quantification (LOQ) of the method were 1 and 5 copies/μL, respectively. The accuracy and applicability of the method were verified using mixed powder samples with known proportions and commercial products.The results confirmed that the developed ddPCR method is highly precise for identifying and quantifying the goose content in food and feedstuffs, indicating the potential applicability in future routine analyses.
