Effects of Different Conditions on the Biofilm Formation of Cronobacter Sakazakii
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Abstract:
The present study investigated biofilm formation of C. sakazakii ATCC 29004 at various growth temperatures (12 ℃, 25 ℃ and 37 ℃), incubation medium (tryptone soya broth and infant formula broth) and surface material (stainless steel, glass and silicone). Firstly, attachment and biofilm formation were evaluated by viable counting method. The micro-structure of biofilm formed by C. sakazakii were confirmed by field emission scanning electron microscope. In addition, the effects of growth temperature and surface type on the biofilm resistance to disinfectants were also determined. The results showed that higher temperatures (25 ℃ and 37 ℃) induced stronger attachment and biofilm formation than 12 ℃, the bacterial biofilm formed better in tryptone soy broth than in infant formula broth. The silicone surface had significantly stronger attachment than stainless steel and glass at 37 ℃, but there was no differences in attachment and biofilm formation on different materials at other conditions. C. sakazakii formed flat and thin biofilms when growing at 12 ℃, but C. sakazakii formed compact and thick biofilms at 25 ℃ and 37 ℃. The biofilm structure were different at three different surface materials. The biofilm resistance to disinfectants is affected by growth temperature, incubation medium and surface type. The results indicated that the growth temperature, incubation medium and surface material were important to control biofilm formed by C. sakazakii.
