High-yield-of-protease Strain Treated by ARTP and Its Improvement of Fermentation of Soybean Meal
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Bacillus amyloliquefaciens 10160 was treated by the technology of atmospheric and room temperature plasma (ARTP). Under the optimum dose of of lethality 80% for ARTP-induced mutagenesis (10 s), two stable mutant strains of C5 and C12 were succefully screened. Compared with the original strain, the neutral protease activities of C5 and C12 strains were increased by 86.0% and 85.0%, respectively. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) observations indicated that, the composition of the proteins extracted from C5, C12, and the original strains was similar, while the treated samples exhibited higher protein concentrations. The liquid fermentation experiments of soybean meal showed that, as compared with the original strain, the yield and content of peptides in the fermentation broth of C5 and C12 strains increased by 14.2% and 15.6%, and 13.2% and 11.8%, respectively. Neutral protease activities in the fermentation broths of C5 and C12 strains increased by 26.00% and 29.84%, respectively. There was no significant difference in the molecular weight distribution and ACE inhibition rate between the strains. Therefore, ARTP treatment of Bacillus amyloliquefaciens 10160 can significantly increase the yield of peptides produced by liquid fermentation of soybean meal.
