Antimicrobial Activity of Natural Antimicrobial Agents against Zygosaccharomyces Rouxii
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The antimicrobial activity of 20 natural antimicrobial agents from plant, animal and microbial sources against Zygosaccharomyces rouxii was investigated in this study. The antimicrobial activity of 20 natural antimicrobial agents was determined based on the diameter of the inhibition zone by the double-layer plate perforated method. The minimum inhibitory concentrations (MICs) of natural antimicrobial agents against Zygosaccharomyces rouxii were determined by the double dilution method. The spectrophotometric method was used to determine the growth of Zygosaccharomyces rouxii in YPD medium and juice, e.g. the effects of two natural antimicrobial agents and different proportions of the two natural antimicrobial agents into juice on the growth of Zygosaccharomyces rouxii, along with the effects of citric acid concentration, temperature and ultraviolet irradiation time on the stability of antimicrobial activity of the composite antimicrobial agents. The results showed that antimicrobial agents had the highest antimicrobial activities against Zygosaccharomyces rouxii. The growth of Zygosaccharomyces rouxii in YPD was better than in juice. Two kinds of microbial inhibitors, natamycin and ε-polylysine, were screened out, and they exhibited synergistic effect. Citric acid concentration, temperature, ultraviolet irradiation time affected the stability of antimicrobial activity of the composite antimicrobial agents.
