Abstract:The synergistic inhibitory effect of complex polysaccharides degradation products from Coprinus comatus, Gracilaria Lemaneiformis and Porphyra haitanensis on HeLa cell proliferation in vitro and its mechanism were investigated. In the experiment, the complex polysaccharides degradation products were combined by three kinds of polysaccharides degradation products from Coprinus comatus, Gracilaria Lemaneiformis and Porphyra haitanensis. The influence of complex polysaccharides degradation products on HeLa cells proliferation was assayed by MTT test. The intracellular level of reactive oxygen species was determined by DCFH-DA and the change of mitochondrial membrane potential was measured by JC-1. Based on orthogonal experiment, when the quality ratio of three kinds of polysaccharides degradation products from Coprinus comatus, Gracilaria Lemaneiformis and Porphyra haitanensis was 4:4:5, the inhibition effect on proliferation of HeLa cells was the strongest. In a concentration of 100 μg/mL, the inhibition rate was 75.72%, which outclassed the maximum inhibition rate of 56.09% when the three kinds of single polysaccharides degradation product were used alone. The results indicated that treatments of complex polysaccharides degradation products could significantly improve the intracellular level of reactive oxygen species and decrease mitochondrial membrane potential by DCFH-DA and JC-1, respectively. Combination of polysaccharides degradation products from Coprinus comatus, Gracilaria Lemaneiformis and Porphyra haitanensis, exhibited syneigistic the inhibition on proliferation of HeLa cells. The effect of complex polysaccharides degradation products might be related to increasing reactive oxygen species and reducing mitochondrial membrane potential.
