Protective Effect of Natural Deep Eutectic Solvent on Green Fluorescent Protein
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Abstract:
Natural deep eutectic solvent (NADES) is a new kind of “green” solvent, which can be applied to the extraction and biocatalysis of natural bioactive substances. In this study, green fluorescent protein (GFP) was used as a model protein for investigations on the protective effects of different NADESs on the stability of GFP at 70 ℃. It was found that the hightest fluorescence retention rate of GFP was in choline chloride sorbital (CS) under heat treatment at 70 ℃. The effect of the water content in CS NADES on the stability of GFP was examined, and CS NADES with a water content of 40% had the highest stability. CS NADES with 40% water content also enhanced the tolerance of GFP against SDS, but didn’t improve significantly the tolerance of GFP. CS NADES also contributed to the improvement of the thermal stability of lipase AOL at 45 ℃. In summary, CS NADESs provided a good protective effect on proteins and have application prospects in the fields of food processing and biocatalysis.
