Importance of the S2P Protease Sll0528 of Synechocystis sp. PCC6803 to Ammonium Stress Tolerance
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Abstract:
Sll0528 is one of the site-2-protease (S2P) in Synechocystis sp. PCC 6803. In the work, the growth of three mutants of Synechocystis (the mutant overexpressed with OE0528 of the sll0528 gene, the ∆sll0528- knockout mutant, and the wild type) was compared under different concentrations of ammonium chloride. The results indicated that the knockout mutant ∆sll0528 was more sensitive to 120 mM ammonium chloride than the wild type, with the photosynthetic system damaged more seriously; at 180 mM ammonium chloride, the growth of wild type was inhibited, while the sll0528-overexpressed mutant OE0528 grew well, which showed higher toleration to the high concentration of ammonium, and better protection for the photosystem pigment phycocyanin and chlorophyll than the wild type. All the results suggested that Sll0528 plays an important role in the acclimation to ammonium stress in Synechocystis sp. PCC6803, probably through protected or recovered the photosystems under ammonium stress directly or indirectly. This is the first report to unveil the importance of S2P protease in ammonium stress of cyanobacteria, thus provding the base for the research of acclimation to ammonium stress and improvement of ammonium tolerance and utilization in microalgae in the future.