Protection of Liver Injury Induced by Carbon Tetrachloride of Kunlun Chrysanthemun by Proanthocyanidon

Abstract:

The protective effect of Kunlun chrysanthemun proanthocyanidin (KCPC) against acute liver injury induced by carbon tetrachloride in mice was investigated. The effects of KCPC on liver appearance pathology and liver index in mice with acute liver injury were analyzed; alanine aminotransferase (ALT) and aspartate aminotransferase (AST) level in serum, as well as antioxidant enzymes SOD, GSH-Px and lipid peroxidase MDA activities in liver of mice were determined by biochemical methods. In addition, the protective effect of KCPC on mitochondria was analyzed by inhibiting mitochondrial membrane swelling induced by Fe2+-Vc and the activity of succinic dehydrogenase (SDH) was evaluated. The results showed that, in the KCPC groups, no distinct pathological changes occurred, while for the control group, the pathology of mice liver tissue dramatically changed, with the activity of ALT and AST in serum increasing remarkably. The activity of ALT and AST in the high-dose KCPC group (800 mg/kg) decreased by 82.5% and 69.4%, respectively. The activities of SOD and GSH-Px increased by 101.1% and 92.1%% (p<0.01), respectively, whereas the MDA content decreased 45.2% (p<0.01). In addition, KCPC has the ability to inhibit the swelling of mitochondria, and the activity of SDH in 400 mg/kg dose group was 1.22 times that of the control group. The results suggest that KCPC exerts a significant protective effect on CCl4 induced liver injury in mice, and its mechanism may be related to the upregulation of antioxidative enzyme activity.