Study on the Flavonoids in the Leaves of Prunus cerasifera Grown in Xinjiang
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Abstract:
This study analyzed the chemical constituents of flavonoids in the active fraction of ethyl acetate in the leaves of Prunus cerasifera. The leaves were firstly soaked in methanol and the alcoholic extract was extracted sequentially using cyclohexane, ethyl acetate and n-butanol. The active fraction of ethyl acetate was roughly separated by MCI (Middle Chromatogram Isolated Gel) macroporous resin column, followed by further purification by chromatography using silica gel column, Sephadex LH-20 gel column, ODS (Octadecyl silane) reverse silica gel column and preparative HPLC. The isolated flavonol constituents were identified by 1H-NMR and 13C-NMR analyses. Identification of compounds based on their physicochemical properties and nuclear magnetic resonance spectroscopy (NMR) data and through comparison with related references. Eleven flavonol flavonoids were isolated and identified: quercetin-3-O-α-L-arabinoside (1), quercetin-3-O-β-D-xyloside (2), quercetin-3-O-β-D-galactoside (3), quercetin-3-O-α-L-rhamnoside (4), kaempferol-3-O-α-L-arabinoside (5), kaempferol-3-O-β-D-xyloside (6), kaempferol-3-O-β-D-glucoside (7), kaempferol-3-O-α-L-rhamnoside (8), trans-dihydrokaempferol-3-O-α-L-rhamnoside (9), cis-dihydrokaemp-ferol-3-O-α-L-rhamnoside (10), cis-dihydrokaempferol-3-O-β-D-glucoside (11). All flavonoids were isolated and identified from the leaves of P. cerasifera for the first time, with quercetin and kaempferol glycosides being the major flavonoids.
