Simultaneous Determination of Vitamins A, D and E in Oil-rich Foods using Gel Permeation Chromatography Coupled with MS
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Abstract:
The vitamins A, D and E in oil-rich foods were simultaneously determined using high performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS), after purified with a gel permeation chromatography (GPC). The optimal extraction conditions were achieved on the PT785 Bio-BeadsS-X3 column, with a cyclohexane - ethyl acetate mixture at ratio of 5:5 (V/V) as the mobile phase. The optimal MS condition was positive electrospray ionization (ESI+)-MS method under multiple reaction monitoring (MRM) mode. The results indicate that retinol, retinol acetate, vitamin D2, vitamin D3, vitamin E were all in good linear relationship with correlation coefficient above 0.999 in the concentration range of 5-200 μg/L; the limit of detection of this method were 2 μg/kg, 1 μg/kg, 5 μg/kg, 2 μg/kg and 10 μg/kg respectively; the recovery rate at four spiked levels range from 93.1 to l06.6% with relative standard deviations (RSD, n = 6)above 1.09% and lower than 4.77%. The method is accurate, sensitive, rapid and easy, and can be applied to determine vitamins A, D and E in oil-rich foods.
