Determination of Sulfonamides and Quinolones in Eggs by QuEChERS EMR-Lipid and High Performance Liquid Chromatography-Tandem Mass Spectrometry
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Abstract:
A sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method has been developed for assaying fourteen sulfonamides and sixteen quinolone antibiotics in egg samples. The egg samples were extracted with 8 mL 2% formic acid acetonitrile, shaked in 0.5 mL 0.1 mol/L EDTA buffer (pH 7). The extract was purified with QuEChERS EMR-Lipid-based rapid sample treatment method, and gradiently eluted with 0.2% formic acid (A) and acetonitrile (B) as the mobile phase. The electrospray ion source was applied with multiple reaction monitoring (MRM) for qualitative and quantitative analysis of 30 sulfonamides and quinolones. The results showed that the recoveries were 50.09%~102.11% for the 30 antibiotics at spiked levels of 8~32 μg/kg, and the relative standard deviation(RSD) was less than 15.66%. The limit of detection (LOD) for the 30 antibiotics was 0.00131~0.09717 μg/kg. The method of determinating sulfonamides and quinolones is simple, easy, and reliable, which can meet the rapid and accurate detection requirements of sulfonamides and quinolones in eggs.
