Determination of Allergens of Antarctic Krill by Ultra-high Performance Liquid Chromatography Coupled with High Resolution Quadrupole Time of Flight Mass Spectrometer
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Abstract:
A method was established in this study to determine allergen of Antarctic krill based on high resolution mass spectrometry. The protein of Euphausia superba is determined by Foline-Phenol and digested by sequence-grade trypsin. The allergens are identified by NCBI protein database and computer retrieval, and validated by using Skyline software combined with triple quadrupole mass spectrometry. Two main kinds of allergens were detected from trpsin digested peptides of Euphausia superba, from which tropomyosin and arginine kinase could be identified. And the corresponding codes of identified peptides of protein allergens were 74 and 44 respectively. As compared with ELISA and PCR, the method is more sensitive, accurate and easy to operate, which may provide a strategy to reduce allergens in foods.
