Crude Protein Extraction from Spirulina Platensis by Ultrasound Coupling Subcritical Water and Its Anti-oxidant Activity
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Abstract:
In this work, ultrasound coupling subcritical water extraction of crude protein from Spirulina platensis was investigated. The extractive reactor was redesigned to increase the contact area of the raw material and subcritical water, and the extraction rate of crude protein was improved significantly from 10.30% to 40.20% (p<0.01); The factors affected on the extraction rate, including temperature, ultrasonic power, pressure and time were systematically discussed using single factor level analysis, and the order was temperature>ultrasonic power>extraction time>pressure; The optimal extraction conditions were obtained by orthogonal design and verification, and the best extraction rate of 74.32% was achieved under the following extraction conditions: temperature of 150 ℃, ultrasonic power of 200 W, extraction time of 80 min and pressure of 10 MPa. The antioxidant activity of crude protein extracted with different temperatures was determined by measuring its reducing power and clearance ability on DPPH and ABST+ and OH- radicals in vitro, and the results showed that crude protein extraction exhibited an excellent antioxidant activity in a dose-dependent manner at concentrations of 0.15~10.00 mg/mL. Therefore, ultrasound coupling subcritical water extraction of protein from marine algae can be used as a new technology with high efficiency, low energy consumption and environmental protection.
