Fermentation Transformed Ginsenoside by Lactic acid bacteria
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Abstract:
Lactic acid bacteria suitable for ginsenosides bioconversion were screened and the fermentation conditions were optimized. The bioconversion pathway of the ginsenosides during the fermentation was discussed. The results showed that Lactobacillus plantarum exhibited the strongest ability to produce rare ginsenosides. Optimal fermentation conditions were 35 ℃, pH 7.0, incubation time of 16 days, 7% inoculum size and 10% substrate concentration. The contents of 3 rare ginsenosides were 106.52 μg/mL(F2), 74.62 μg/mL(Rg3) and 100.56 μg/mL(CK), which were enhanced 188.13%, 203.21% and 395.86%, respectively. The changes in major ginsenosides content were consistent with the possible transformation pathway of ginsenosides. The results indicated that the ginsenosides were converted to rare ginsenosides through Lactic acid bacteria fermentation and the reasonable fermentation process was constructed to improve the content of rareginsenosides. This work has important significance for microbial transformation of ginsenoside, and the fermentation broth have an advantage in deep-processing ginseng products.
