Lipase-catalyzed Synthesis of Cinnamate Sucrose Ester and Its Antibacterial Activity
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Abstract:
A feasible route for lipase-catalyzed synthesis of sucrose ester with excellent antibacterial properties was developed in this paper. Binaryorganic solvent of DMAc and tert-amyl alcohol was used as the reaction medium to well dissolve the polar sugar substrate and maintain the enzyme activity of immobilized lipase. And the effect of solvent volume ratio and reaction temperature on the synthesis method was explored. The FTIR of ester product showed that the lipase promoted the acylation of sucrose with cinnamic acid. The structure of ester product was further identified by 13C-NMR spectrum and it confirmed that esterification occurred on the C-6 cite of sucrose. TGA analysis showed a high thermal stability of cinnamate sucrose ester. And the antimicrobial activity of cinnamate sucrose ester was determined, the inhibitory rates of the ester product to Escherichia coli and Geotrichum candidum were 97.83% and 96.04%, respectively. Furthermore, the cinnamate sucrose ester showed great water retention property and the water retention rate was 76%. The findings are of importance for the application of sucrose ester in food and cosmetics industry.
