In Vitro Antioxidant Activities of Nervilia Fordii Methanol Extracts
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Abstract:
The in vitro antioxidant activities of Nervilia fordii mthanol extracts (NFME) were investigated. Total polyphenols and flavonoids in NFME were determined by ultraviolet spectrophotometry. The antioxidant capability of NFME was evaluated according to the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals(•OH) scavenging assay, respectively. Protective effect of NFME was investigated using a model of hydrogen peroxide (H2O2, 150 μmol/L) induced oxidative stress in intestinal epithelial Caco-2 cells. Cell viability was determined by MTT assay. The cellular levels of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were determined by commercial assay kits according the manuscripts. The cellular levels of malondiadehycle (MDA) and reactive oxygen species (ROS) were determined by thiobarbituric acid reactive substance (TBARS) and dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay, respectively. The levels of interlukin-8 (IL-8) and IL-10 were determined used an enzyme linked immunosorbent assay (ELISA). The NFME were enriched in total flavonoids (4.72 ± 0.13 mg Rutin/g) and poly phenols (4.25 ± 0.46 mg GAE/g). NFME also exhibited a great DPPH and ?OH radical scavenging activity. NFME was able to increase the cell viability, and significantly increased the activity of SOD, CAT and GSH-Px in oxidative damaged Caco-2 cells. In addition, NFME treatment was also effectively decreased the generation of ROS, MDA and IL-8 secretion in H2O2 treated Caco-2 cells. In contrast, NFME increased the IL-10 secretion in damaged Caco-2 cells. These results suggested that the NFME exhibits a great in vitro antioxidant activity and can effectively attenuate the H2O2-induced oxidative stress in Caco-2 cells.
