Abstract:In this work, to investigate the coagulation pathogenicity of porcine Staphylococcus aureus to different animal hosts and whether they harbor staphylococcal coagulase genes of vwbC, vwbSbo4, vwbSov2 and vwbSeq1, 130 S. aureus were isolated from swine farm, slaughterhouse and terminal market in Xiamen. Coagulation adaptability of these isolates to rabbit, bovine and goat plasma were studied by coagulase assays. The distribution of vwbC, vwbSbo4, vwbSov2 and vwbSeq1 genes in 130 S. aureus were also investigated by PCR amplification. The results showed that positive coagulation rate of 130 S. aureus to rabbit, bovine and goat plasma were 100.00%, 28.46% and 28.46%, respectively. The staphylococcal coagulasegenes of vwbC, vwbSbo4 and vwbSov2 were identified in 100.00% (130/130), 26.92% (35/130) and 26.93% (38/130) of all isolates, respectively. None of isolates harbored vwbSeq1gene.The detection rates ofvwbSbo4 and vwbSov2 genes and the positive coagulation rates of bovine and goat plasma of S. aureus isolated from pig farms were significantly higher than those isolated from slaughterhouse and terminal marker isolates (p<0.05). This work showed that porcine S. aureus possessed coagulation pathogenicity to different animal hosts and provided a basis for studying on the coagulation pathogenicity and host adaptability of porcine S. aureus.
