Immunomodulatory Effect of Polysaccharide Isolated from Cinamomum camphora Fruits on Macrophage RAW264.7
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Abstract:
Cinamomum camphora is proved to possess a lot of biological activities, including antifungal, antioxidant, antibacterial, anti-allergic, and anti-inflammatory activities. However, little research has been reported on the C. camphora fruit polysaccharides (CCFP). Therefore the objective of this study was to isolate polysaccharide from C. camphora and evaluate its immunomodulatory activities on Macrophage RAW264.7. The results indicated that CCFP significantly promoted the production of intracellular nitric oxide (NO) and cytokines such as prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) in RAW 264.7 cells. When the dosage of CCFP was 100 μg/mL, the secretion of NO, TNF-α and PGE2 reached to the maximum values of 53.53 ± 4.25 mu M, 1008.32 ± 35.23 pg/mL and 434.56 ± 25.23 pg/mL, respectively. The result of RT-PCR analysis indicated that CCFP also enhanced inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-α expression. Further analyses demonstrated that CCFP rapidly activated the extracellular signal-regulated kinase (ERK) and enhanced the transcriptional activities of activator protein-1 (AP-1) and nuclear factor (NF)-κB via toll-like receptor 4 (TLR4). Taken together, these results suggest that CCFP can improve immunity, and could be explored as a potential immunomodulatory agent in functional food.
