Comparative Analysis of the Equivalence of Filter Membrane Method and Plate Counting Method in Microbiological Examination of Filterable Liquid Beverages
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Abstract:
The aim of this study was to investigate whether there is equivalence between filter membrane method and plate counting method in examination of microbiological indexes of filterable beverage which include total numbers of colony and the count of mold, yeast and coliform. Through pre-experimental results, 100 copies of filterable liquid beverage were collected and then added with quantitative bacterial fluid for examination of total numbers of colony and the count of mold, yeast and coliform by using filter membrane method and plate counting method respectively. After that, comparative analysis on results of the two methods was conducted based on ISO 17994:2014 "Water quality- Requirements for the comparison of the relative recovery of microorganisms by two quantitative methods ", so that the equivalence of the two methods in microbiological examination of filterable liquid beverage could be verified. The results indicated that, filter membrane method and plate counting method have equivalence in microbiological examination of filterable liquid beverage when its sampling quantity reaches 100. Thus, the two methods are alternative when applied to the examination of microbiological indexes of filterable liquid beverage.
