Determination of Tetracycline Antibiotic Residues in Chicken by Molecularly Imprinted Solid Phase Extraction Coupled with High Performance Liquid Chromatography
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Abstract:
A selective molecularly imprinted polymer for separating tetracycline antibiotics was synthesized via bulk polymerization using tetracycline hydrochloride as the template, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker and azobisisobutyronitrile as an initiator. This synthesized material was used as the solid phase adsorbent that was coupled with high performance liquid chromatography (HPLC) to establish a solid phase extraction-HPLC system for the determination of trace residues of three tetracycline antibiotics in chicken meat. The obtained results showed that the material has high adsorption capacity and specific recognition. A good linearity of the three tetracycline antibiotics in the range of 0.1-1.0 mg/L (R2 > 0.99) was detected, with the minimum detection limits of oxytetracycline, tetracycline, doxycycline as 0.13 μg/L, 0.12 μg/L, 0.14 μg/L, respectively, and relative standard deviation (RSD) values of the five replicates as 1.27%, 1.31%, 1.94%, respectively. Whentetracycline antibiotics were added to chicken at three different concentrations (50 μg/kg, 100 μg/kg and 200 μg/kg, respectively), the recoveries were 88.33%~94.95%, 80.94%~88.02%, and 87.69%~93.08%, respectively, with corresponding RSD values being 2.08%~3.63%, 1.46%~2.37%, and 1.04%~3.03%, respectively. The results of this study show that the currently established method can be used for the detection of tetracycline residues in chicken meat.
