Study on the Preparation of Collagen Peptide from Tilapia Skin
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Abstract:
The collagen peptides from tilapia skin has been extracted by using an enzymatic method. The composition of tilapia skin was also analyzed. A novel continuous phase-transition extraction method at low temperature was used to remove fat from tilapia skin. The degree of hydrolysis, molecular weight and organoleptic investigation were used as indicators to study the effects of protease types on the hydrolysis. Response surface experiment was designed to optimize the preparation technology of the collagen peptide. GFC was used to determine the molecular weight distribution of hydrolysates. The results showed that alcalase was the best enzyme for the hydrolysis, and the optimal hydrolysis conditions were determined as follows: enzymolysis time 7 h, alcalase dosage 7%, temperature 60 ℃, and the pH 10.38. Under these conditions, the maximum hydrolysis degree was 23.01%. The peptides obtained is mainly composed of peptide with 2 to 8 amino acid residues, and has good physiological activity. It could be widely used as a functional factor in various industries, such as health food, cosmetics, medicine and other industries.
