Effects of Storage Conditions on the Content and Antioxidant Activity of Polyphenols from Picea koraiensis Nakai Seed Scales
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Abstract:
In this experiment, the effect of storage conditions, such as pH, light, temperature, metal ions and additives, on the content, anti-oxidative capacity and stability of polyphenol in the cone scale of Picea koraiensis Nakai were researched. The polyphenols in Picea koraiensis Nakai cone scale showed the highest stability with the content rates of 93.08% at 4℃ and relatively high stability in darkness with content rates of 87.23%. under these two conditions, the DPPH scavenging rates of phenol were 80.65% and 82.16%, respectively, and the total reducing capacity (OD value) of polyphenols in Picea koraiensis Nakai cone scales were 0.854 and 0.798, respectively. The effects of pH on these three parameters indicated that acidic conditions were more compatible than basic ones. The content rate, DPPH scavenging rate and total reducing capacity of the polyphenols at pH2 were 2.21, 4.56, and 2.78 times, respectively, than those at pH 10. Ions showed different effects depending on the kinds. The ions Na+, K+, and Ca2+ showed slight effect, while Cu2+, Zn2+, and Fe3+ had significant impacts on the polyphenols content and anti-oxidative ability. In terms of additives, sucrose solution with concentration higher than 30% and 0.01% sodium benzoate solution could retard the degradation of polyphenols in the cone scale, while their effects on the anti-oxidative ability were not significant. This research will provide a new prospect for the application of Picea koraiensis Nakai cone scale polyphenols.
