Effect of FAS2 Gene Overexpression on the Production of Favor-active Esters in Saccharomyces cerevisiae
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Abstract:
The effects of fatty acid synthase 1 gene (FAS1) and fatty acid synthase 2 gene FAS2 overexpression on medium cahin fatty acid ethyl esters (MCFAEEs) in Saccharomyces cerevisiae were investigated. Previous experiment showed that FAS1 gene-expression strains, α5F-FAS1 and α5O-FAS1, could improve the production of MCFAEEs in S. cerevisiae. The 154.31% and 65.22% improvement of ethyl caproate and ethyl octanoate production and 23.53% increasement of ethyl caprate accumulation were observed in the Co-overexpression of FAS1 and FAS2 with FAA1 deletion engineered strain FF1F2 compared to strains α5F-FAS1. Consistent with this effect, the Co-overexpression strain OF1F2 achieved 17.34%, 30.71% and 27.12% increasement of the three substances mentioned above and 28.13% improvement of ethyl dodecanoate, respectively, compared to strains α5O-FAS1. The results showed that FAS1 and FAS2 gene co-overexpression could further enhance the yield of MCFAEEs in S. cerevisiae. At the same time, we also found that overexpression of FAS1 gene can improve the production of ethyl acetate. To explore the preliminary machnism of this phenomenon, the relative expression level of Acetyl-CoA related genes was measured by RT-PCR. The results showed that the increase in the amount of ethyl acetate produced by FAS1 overexpression is mainly attributed to the up-regulation of ATF1 gene expression.
