Characterization of the Prevalence and Distribution of Lsa(E) gene in Staphylococcus aureus Isolated from Pig Farms
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Abstract:
The spread of foodborne multi-resistant bacteria poses a serious threat to food safety and public health. The lsa(E) gene confers resistance to lincosamides, pleuromutilinsand streptogramin A. In order to investigate the prevalence and distribution oflsa(E) gene and multi-resistance gene clusters inStaphylococcus aureusisolated from pig farms in Xiamen. The lsa(E) gene and the genetic environment were tested by polymerase chain reaction (PCR) method. Then, lsa(E) positive S. aureus were characterized by drug susceptibility testing. In total, gene cluster: aadE-spc-lsa(E)-lnu(B)-tnp was found in 28 out of 29 lsa(E) positiveS. aureus. Resistance was most frequently observed to clindamycin(100.0%), clarithromycin(100.0%) followed by trimethoprim/sulfamethoxazole(96.6%), tetracycline(96.6%), ciprofloxacin (96.6%), and gentamicin (72.4%). All the isolates were susceptible to oxacillin, quinupristin-dalfopristin, and linezolid. The multidrug resistance rate was 100.0%, and the main resistance profiles of S. aureus strains was PEN-GEN-TET-CLA-SXT-CLI-CIP-MXF. This study provides a theoretical basis for the prevention and treatment the food quality and safety problem caused by foodborne pathogenic bacteria and the controlling of multi-resistant strains spread of S. aureus in the food chain.
